Laboratory for serology and molecular diagnostics

Name says it all, in this laboratory two types of techniques are applied for diagnostics of infectious diseases in animals. Regarding serology following methods are used for routine diagnostics: fast agglutination, slow agglutination, haemagglutination, inhibition of haemagglutination, ELISA (indirect and competitive), CFT and agar-gel immunodiffusion test. These methods are used for diagnosis of following diseases: brucellosis, Q fever, chlamydiosis, bovine viral diarhhoea, blue tongue disease, enzootic leucosis, equine infectious anemia, IBR/IPV, salmonellosis etc.

Regarding molecular serology conventional PCR and real-time PCR are used as fundamental techniques for diagnostics and genetic sequencing of isolates and amplificates from different types of pathogens is also performed. In this part of laboratory routine diagnostics of brucellosis (detection and differentiation of species, differentiation of Brucella melitensis from Rev-1 vaccinal strain), avian influenza (matrix gen, H5 and H7 subtypes), classical swine fever, bovine viral diarhhoea, Q-fever, blue tongue disease etc.