– Genetics (sire selection, management, nutrition)<\/p>\n
– Semen collection<\/p>\n
– Semen evaluation<\/p>\n
– Semen processing<\/p>\n
– Cryopreservation<\/p>\n
– Quality control<\/p>\n
– Geneticly superior progeny proven sires:<\/p>\n
– Semex Alliance (Canada)<\/p>\n
– Alta Genetics (Canada)<\/p>\n
– Preska (Slovenia)<\/p>\n
– Krnjaca (Srbija)<\/p>\n
– Permanent quarantine system<\/p>\n
– Regular health control<\/p>\n
– Environment in compliance and according the regulations listed in Animal Protection and Welfare Law (Official Journal of Republic of Macedonia 113\/2007)<\/p>\n
– Quality and health control of the feedstuffs entering the facilities<\/p>\n
– Quality and health control of the water entering the facilities<\/p>\n
– Controled vitamine and mineral additives for stimulation of the spermatogenesis<\/p>\n
– No additives of animal origin, listed as specific risk material, accordint the Reg. 1774\/2002<\/p>\n
– Mounting on natural phantom (other animal)<\/p>\n
– Artificial vagina (Hannover type)<\/p>\n
– Preheated at 42 \u030a\u0421<\/p>\n
– Lubrificated with the extender<\/p>\n
– Macroscopical evaluation<\/p>\n
– Microscopical evaluation<\/p>\n
– Phase-contrast microscope (light field \u2013 Phase 1)<\/p>\n
– Magnification 10 \u2013 40 \u0445<\/p>\n
– Preheated stage at 39\u00b0\u0421<\/p>\n
– Microchamber for fresh semen evaluation<\/p>\n
– Volume<\/p>\n
– Color<\/p>\n
– Consistency<\/p>\n
– Presence of foreign artifacts<\/p>\n
– Concentration: > 9 \u0445 103\/ml<\/p>\n
– Proportion of motile spermatozoa: > 70%<\/p>\n
– Absence of increased number of epithelial cells, leucocytes, erythrocytes<\/p>\n
– Absence of foreign origin particles<\/p>\n
– Dilution (Suspension)<\/p>\n
– Microscope evaluation after the dilution<\/p>\n
– \u0421\u0410\u0405\u0410 evaluation \u0438 and determination of the final concentration<\/p>\n
– Packing and labeling<\/p>\n
– Equilibration<\/p>\n
– \u0410ndromed\u00ae (Minitub, Germany)<\/p>\n
– Free of compounds from animal origin (according Reg. 1774\/2002)<\/p>\n
– Primary diludion ratio 1:1<\/p>\n
– Dilution up to ratio 1 : 10<\/p>\n
– Microscope evaluation<\/p>\n
– HTM IVOS\u00ae (Hamilton Thorne Research, USA)<\/p>\n
– Determination of the ratio of the final dilution<\/p>\n
– Recording of the exach concentration of the ejaculate<\/p>\n
– Recording of the proportion of motile spermatozoa<\/p>\n
– Determination of the qualitative motility parameters of the spermatozoa<\/p>\n
– MPP 133 Combo Jet (Minitub, Germanu)<\/p>\n
– 0,5 ml medium-straws<\/p>\n
– Labeling of the straws: sire\u2019s name, ID No, designation of the AI centre, batch No.<\/p>\n
– Cooling to +4\u00b0\u0421<\/p>\n
– Equlibration period 2 \u2013 2,5 hours<\/p>\n
– Automatic MT freezer for semen straws<\/p>\n
– from 20 to 4\u00b0\u0421 in 10 min, temperature slope -1,6\u00b0\u0421\/min<\/p>\n
– at 4\u00b0\u0421 \u2013 equilibration in period of 2 hours<\/p>\n
– from 4\u00b0 to – 6\u00b0\u0421 in period of 2 min., temperature slope – 5\u00b0\u0421\/min<\/p>\n
– at -6\u00b0\u0421 \u2013 stabilization in period of 1 min. crystallization – solidification<\/p>\n
– from -6\u00b0\u0421 to -20\u00b0\u0421 in period of 1 min, temperature slope -14\u00b0\u0421\/min, to compensate the heath release during the crystallization<\/p>\n
– from -20 to -140\u00b0\u0421 \u0421 in period of 4 min, temperature slope -30\u00b0\u0421\/min<\/p>\n
– from -140\u00b0\u0421 to -196\u00b0\u0421 Direct submerging in LN2. (Mueller-Schlosser et al. 2000)<\/p>\n
– Thawing (39\u00b0\u0421, at least 30 sec.)<\/p>\n
– Microscope evaluation (criteria > 50% motile spermatozoa)<\/p>\n
– \u0421\u0410\u0405\u0410 assessment and recording of the obtained results<\/p>\n
Production – Genetics (sire selection, management, nutrition) – Semen collection – Semen evaluation – Semen processing – Cryopreservation – Quality control Sires selection – Geneticly superior progeny proven sires: –…<\/p>\n","protected":false},"author":1,"featured_media":0,"parent":753,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_editorskit_title_hidden":false,"_editorskit_reading_time":0,"_editorskit_is_block_options_detached":false,"_editorskit_block_options_position":"{}","footnotes":""},"class_list":["post-876","page","type-page","status-publish","hentry"],"yoast_head":"\n